Vol. 43

Title:
Strategies in Commercial Micropropagation

Author:
R.A. de Fossard

pp: 261-266

Abstract:
I can trace my learning curve in micropropagation from 1961 and now I can pose questions such as "What is important to the achievement of a successful micropropagation operation? Where should I direct my research?" In a few words, the answer is "to the achievement of high success rates in the acclimatization stage".

In this paper I will be concentrating on my experiences with culture media and my current approach to the selection of media And to start at the end product I will present my latest basal medium and ideas for using it (Table 1) The medium consists of a micronutrient section, a growth factor section, and a gelling agent. Macronutrient, auxin, cytokinin, and carbohydrate sections are treated as experimental variables. Full strength macronutrients are listed in Table 2—and these are diluted (either in groups or as individual components) to prepare different concentrations to suit different purposes. Types of auxins, cytokinins, and carbohydrates and their concentrations are

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