Vol. 43

Title:
Effects of Reduced Humidity and Paclobutrazol on Acclimatisation of Tissue-Cultured Plants

Authors:
Kerrin P. Henderson, Acram M. Taji and Richard R. Williams

pp: 97-102

Abstract:
INTRODUCTION

Plant tissue culture is now a widely used propagation method Its main advantage is the ability to rapidly multiply selected cultivars and to produce genetically uniform plants. The techniques range from simple micropropagation using a bud, node or leaf segment, to cell or protoplast culture. Commercial production mainly involves micropropagation. Whatever the technique used, the final step involves the transfer of the cultured plants from the protected environment of the culture vessel to the nursery bench. The process of the plant adapting to this change in environment is known as acclimatization.

Under in vitro conditions of low light intensities and in the presence of carbohydrate in the medium, plants are heterotrophic i.e., they are unable to photosynthesise to meet their carbohydrate requirements. The high humidity in the culture vessel also results in plants unable to control water loss through transpiration (Brainerd and Fuchigami, 1981; Capellades et al, 1990;

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