ISSN 1611-4426 print and 1611-4434 online | © ISHS 2022 | European Journal of Horticultural Science | Original article
Onion AcCOP10 ectopia overexpression regulates flowering and seedling photomorphogenesis in Arabidopsis
Lei Qin1,2, Yang Xu1,2, Jie Sheng1,2, Qingzhe Yin1,2, Hongzhi Xu1,2, Yifan Geng1,2 and Yong Wang1,2
1 Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs/Northeast Agricultural University, Harbin, P.R. China
2 Department of Agricultural Economics, Faculty of Agriculture and Natural Resources, Ardakan University, Ardakan, Iran
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SUMMARY
Onion (Allium cepa L.) is a biennial vegetable crop response to the appropriate photoperiod in flowering and bulb forming. COP10 (CONSTITUTIVE PHOTOMORPHOGENIC 10) is a member of ubiquitin E2 variant (UEV) protein family, which is forming a complex with damage binding protein (DDB1) and de-etiolated 1 (DET1) to regulate plant flowering and seedling development. In this study, we cloned AcCOP10 from onion and the role of AcCOP10 in flowering regulation and seedling photomorphogenesis were studied using AcCOP10-overexpression transgenic lines in Arabidopsis. The cDNA of AcCOP10 was 531 bp, encoding a 176 amino acid protein, which contains conserved regions of UEV protein. Tissue expression pattern analysis preformed that AcCOP10 had the highest expression in the leaf sheath after bolting. Overexpression of AcCOP10 delayed the flowering time of the wild type and cop10 mutant in Arabidopsis. Transcript levels analysis showed expression of AtCO and AtFT decreased in transgenic lines. Moreover, AcCOP10 compensated the extreme phenotype of cop10 in hypocotyl development in the dark. The expression of ELONGATED HYPOCOTYL 5 (AtHY5) was suppressed when AcCOP10 overexpressed. These results predicted that AcCOP10 might involve in flowering regulation and seedling photomorphogenesis. Keywords onion, AcCOP10, overexpression, flowering, seedling morphogenesis, Arabidopsis |
Significance of this study
What is already known on this subject?
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E-mail: yongwang@neau.edu.cn
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Received: 15 April 2021 | Accepted: 22 June 2021 | Published: 9 September 2022 | Available online: 9 September 2022
