Eur.J.Hortic.Sci. 82 (5) 232-238 | DOI: 10.17660/eJHS.2017/82.5.2|
ISSN 1611-4426 print and 1611-4434 online | © ISHS 2017 | European Journal of Horticultural Science | Original article
Night temperatures affect fruit coloration and expressions of anthocyanin biosynthetic genes in 'Hongro' apple fruit skins
Suhyun Ryu1, Hyun-Hee Han1, Jae Hoon Jeong1, YongHee Kwon1, Jeom Hwa Han1, Gyung Ran Do1, In-Myung Choi1 and Hee Jae Lee2,3
1 Fruit Research Division, National Institute of Horticultural and Herbal Science, Wanju, Korea
2 Department of Plant Science, Seoul National University, Seoul, Korea
3 Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Korea
We analyzed the fruit skin coloration and expressions of anthocyanin biosynthetic genes to determine the effects of night temperature on Hongro apple (Malus domestica Borkh.). Trees were grown under low night temperatures (LNT; 3°C lower than the control) and high night temperatures (HNT; 3°C higher than the control) for 2 months from July to August. LNT promoted an increase of the a* value and decreases of the L*, b*, and h° values in fruit skins, but HNT produced the opposite results. Anthocyanins accumulated in fruit skins with increasing days after full bloom. The anthocyanin biosynthesis was promoted by LNT, but inhibited by HNT. Quantitative real-time polymerase chain reaction analysis revealed that LNT increased the expressions of anthocyanin biosynthetic genes, MdCHS, MdF3H, MdDFR, MdANS, and MdUFGT, but HNT decreased them. These results suggest that temperatures at night affect anthocyanin accumulation in apple fruit skins by regulating expressions of the anthocyanin biosynthetic genes.
anthocyanidin, CIE color scales, fruit quality, high night temperature, transcription factors
Significance of this study
What is already known on this subject?
What are the new findings?
Apples grown at high temperature during fruit development are poorly colored, while those grown at low temperature are well colored. Coloration of apple fruit skins is determined by their anthocyanin contents. The anthocyanin accumulation in apple fruit skins is inhibited by high temperatures under light conditions.
What is the expected impact on horticulture?
Anthocyanin accumulation in apple fruit skins was promoted by low temperature at night, but suppressed by high temperature. The reduced anthocyanin accumulation caused by high temperature at night was due to the decreased expressions of anthocyanin biosynthetic genes, MdCHS, MdF3H, MdDFR, MdANS, and MdUFGT. These results suggest that temperatures at night affect anthocyanin accumulation in apple fruit skins by regulating expressions of the anthocyanin biosynthetic genes.
As a result of global warming, not only high temperatures in the daytime but also tropical night high temperatures are increasing. Our results provide fundamental information for controlling the fruit skin coloration of apples under different temperatures at night.
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Received: 24 February 2017 | Accepted: 25 April 2017 | Published: 23 October 2017 | Available online: 23 October 2017