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  Eur.J.Hortic.Sci. 81 (4) 219-226 | DOI: 10.17660/eJHS.2016/81.4.5
ISSN 1611-4426 print and 1611-4434 online | © ISHS 2016 | European Journal of Horticultural Science | Original article

Polyploidy induced by colchicine in Dendranthema indicum var. aromaticum, a scented chrysanthemum

Miao He1, Wenjie Gao1, Yaohui Gao2, Yingzhu Liu1, Xue Yang1, Hongbin Jiao1 and Yunwei Zhou1
1Department of Landscape Architecture of Northeast Forestry University, Harbin, Heilongjiang, China
2Beijing Forestry University

Dendranthema indicum var. aromaticum from the genus Chrysanthemum is a rare species with a rich flavor. The aim of this study was to find a suitable treatment combination that would effectively induce polyploidy of diploid Dendranthema indicum var. aromaticum and to lay the foundation for the late cultivation of aromatic Chrysanthemum. In this study materials, colchicine concentration and treatment duration were examined for improving the induction of polyploidy. The combinations of three materials (shoot tips, pre-germinated seeds and grin), five colchicine concentrations (100, 200, 500, 1,000 and 2,000 mg L-1) and three treatment durations (12, 24 and 48 h) were tested in Dendranthema indicum var. aromaticum. A total of 7 tetraploids and 301 chimeras determined by chromosome number analysis were obtained. The treatment of grin seeds with 1,000 mg L-1 colchicine for 24 h (14.5%) and shoot tips with 1,000 mg L-1 colchicine for 7 d (40%) were suitable for induction of chromosome doubling. The tetraploid plants displayed much larger stomata with lower density, as well as a higher chloroplast count than the diploid paints. Moreover, tetraploid plants developed larger, thicker leaves, greater flower diameter, more epidermis hairs and shorter plant height than the diploid plants.

Keywords Colchicine, Dendranthema indicum var. aromaticum, induction, identification, polyploid

Significance of this study

What is already known on this subject?

  • Dendranthema indicum var. aromaticum is characterized by a special scent, which is an important source of aroma in genus Dendranthema. Hybridization is the good way to produce new scented chrysanthemum. However, it is difficult to cross breed if one species is a diploid while the other is a higher ploidy level. Therefore, we decided to double the somatic chromosomes of D. indicum var. aromaticum, in order to increase the success of crosses between the two species.
What are the new findings?
  • In this study, the tetraploid plants displayed much larger stomata with lower density, as well as a higher chloroplast count, than the diploid plants. Moreover, tetraploid plants developed larger, thicker leaves, greater flower diameter, more epidermis hairs and shorter plant height than the diploid plants.
What is the expected impact on horticulture?
  • We had got the tetraploids of Dendranthema indicum var. aromaticum, which were important parents for scented Chrysanthemum. Later, we will cross between the tetraploid and cultivated Chrysanthemum and get the scented Chrysanthemum, which has important significance for improving the ornamental traits of horticultural plants.

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Received: 22 March 2016 | Accepted: 1 July 2016 | Published: 29 August 2016 | Available online: 29 August 2016

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