Volume 48 Number 3 Article 8 Pages: 173-175
Year 1994 Month 7
Title: Culture Date and Germination Procedure Affects Success of Nectarine Ovule and Embryo Culture
Authors: J.X. Chaparro and W.B. Sherman
Citation
Abstract:
Immature 'Sunlite' nectarine ovules were
grown on Knop's ovule growth medium for 30
days and germinated following stratification
either in vitro on a modified Knop's germination
medium or in moist perlite.
The minimum age
at which any embryos were successfully ovule
cultured and germinated was 53 days after full
bloom.
Germination percentage increased significantly at 60 and 67 days after full bloom.
Germination percentage in perlite was not great
ly different from that of ovules cultured in vitro
on modified Knop's germination medium, but
the 53 and 60 days after full bloom embryos
germinated in perlite were visibly weaker.
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