Volume 47 Number 4 Article 2 Pages: 185-192
Year 1993 Month 10
Title: Changes in Levels and Isozymes of Peroxidase in Wounded Peach Bark
Authors: M.M. Goodin, A.R. Biggs and A.M. Castle
Citation
Abstract:
Bark on one-yr-old branches from six peach
cultivars was mechanically wounded then exam
ined over time for changes in peroxidase iso
zymes, total peroxidase activity, and total pro
tein.
Four peroxidase isozymes were identified
and changes in total activity were associated
mostly with one isozyme.
Changes in peroxidase
activity in response to wounding were observed
with activity increasing to the highest levels at 4
and 7 days post-wounding.
At 10 and 14 days
post-wounding, peroxidase levels had returned
to levels similar to the control.
An unidentified
would response protein was detected in these
experiments.
The timing of the appearance of
this protein was correlated with the known
relative susceptibility of the cultivars to the
peach canker pathogens, Leucostoma persoonii
and L. cincta. Comparisons of the peroxidase
isozyme profiles and total peroxidase activity
among cultivars provided no indication that
peroxidase profiles could be used as a marker
for resistance to the peach canker pathogens.
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