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  Eur.J.Hortic.Sci. 80 (3) 134-138 | DOI: 10.17660/eJHS.2015/80.3.6
ISSN 1611-4426 print and 1611-4434 online | © ISHS 2015 | European Journal of Horticultural Science | Original article

Preliminary identification of pear accessions of 'Lubeničarka' group using RAPD markers

M. Kajkut1, G. Đurić1,2 and N. Mićić1,2
1 Genetic Resources Institute
2 Faculty of Agriculture
University of Banja Luka, Banja Luka, Republic of Srpska, Bosnia and Herzegovina

The preliminary identification of six pear (Pyrus communis L.) accessions of the ‘Lubeničarka’ group in the collection of the Republic of Srpska Gene Bank was done in order to compare their genetic similarity. Pear accessions were analysed using Randomly Amplified Polymorphic DNA (RAPD) markers. Pear accessions of the ‘Lubeničarka’ group: ‘Lubeničarka’, ‘Lubeničarka Milići’, ‘Lubeničarka Zvornik’, ‘Lubeničarka Vlasenica’, ‘Krupna Lubeničarka’ and ‘Crna Lubeničarka’, were analyzed using eleven primers which amplified base pairs of the length between 400 and 2,000 bp. The obtained results showed that all accessions can be separated into two groups. The first group are accessions with the coefficient of similarity (1.0), namely ‘Lubeničarka’, ‘Crna Lubeničarka’, ‘Lubeničarka Milići’, ‘Lubeničarka Vlasenica’ and ‘Lubeničarka Zvornik’. Only one accession belongs to the second group, namely ‘Krupna Lubeničarka’ accession with genetic similarity coefficient of 0.6.

Keywords field collection, genetic characterization, genetic similarity, germplasm, duplicates

Significance of this study

What is already known on this subject?

  • There is a number of pear genotypes with red flesh of fruit under common name ‘Lubeničarka’ in Bosnia and Herzegovina. The pomological characterization done on three genotypes showed clear divergence of one genotype (‘Krupna Lubeničarka’) in relation to other two which also showed some but not clear morphological differences.
What are the new findings?
  • Six pear genotypes from ‘Lubeničarka’ group analysed by RAPD markers showed that regardless on morphological variability, this population is classified into two main groups, ‘Krupna Lubeničarka’ with clear pomological characteristics and five genotypes in group ‘Lubeničarka’ with the same coefficient of genetic similarity.
What is the expected impact on horticulture?
  • Recognition of genotype ‘Krupna Lubeničarka’ as varieties with stable pomologic and genetic traits. Elimination of duplicate accessions from field Gene Bank.

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Received: 10 October 2014 | Revised: 9 January 2015 | Accepted: 6 March 2015 | Published: 17 June 2015 | Available online: 17 June 2015

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