Volume 65 Number 4 Article 2 Pages: 185-191
Year 2011 Month 10
Title: Formation of Graft Union in Pear: A Histological Study by Using Light Microscopy
Authors: C. Zlati, G. Grădinariu, M. Istrate and L. Draghia
Citation
Abstract:
This research was carried out at the Experimental Farm of the University of Agricultural Sciences and Veterinary
Medicine Iasi, Romania during 2008-2010. The anatomical structure of the graft union in heterograft combinations
of four pear variety scions on quince rootstock was studied.
Tissue samples were taken from graft unions
six to twelve months after grafting and first fixed in ethyl alcohol then in formalin/glacial acetic acid solution.
Observations were made of the callus tissue between rootstock and scion, the necrotic layers that were formed,
the formation of new cambium tissue between rootstock and scion, cambial continuity and the formation of new
vascular tissues in the cambium.
New cambium, xylem and phloem tissues were formed early in the region of the
graft union but a longer time was needed for continuous connections to form within the cambium.
Abnormalities
were seen within the area of the graft union with some of the scion/rootstock combinations indicating incompatibility
for these particular graft combinations.
The results provide the possibility of applying an early selection
method that will help to predict the future compatibility level of a specific scion/rootstock combination long before
any external symptoms could be observed.
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