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Abstract: The maintenance of stable cultures of Agaricus bisporus is primarily the responsibility of commercial spawnmakers and the spawn plants of some larger mushroom farms. Most individual mushroom growers depend on the knowledge and skill of spawnmakers to provide reliable spawn. A few noncommercial culture collections are maintained such as the extensive collection of the Mushroom Research Center at The Pennsylvania State University. It has been possible for these agencies to maintain over extended periods of time cultures which produce mushrooms excellent in quantity and quality by paying careful attention to the preparation of suitable media, selection of mycelium for transfer which appears to be normal phenotypically, incubation of cultures under optimum environmental conditions, and skillful transferring of cultures at reasonable intervals of time (KLIGMAN, 1943; KNEEBONE, 1965, 1968; KNEEBONE et al., 1972; LAMBERT, 1959). In recent years, the use of ultra-low temperature for freezing and storing fungus cultures has become feasible (HWANG, 1968), and preliminary results using cryogenic techniques with A. bisporus are promising (HWANG and SAN ANTONIO, 1972). In view of these results a 10-year cooperative research project has been undertaken between the American Type Culture Collection and The Pennsylvania State University to further determine the applicability of cryogenic techniques to the preservation of stock cultures of the cultivated mushroom, A. bisporus. Results of the first several years of this joint effort are reported herein.
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