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Abstract: Pure cultures of Agaricus bisporus do not normally form primordia on agar media and this has limited progress in nutritional investigations which are relevant to the formation of mushroom fruit-bodies (the crop). Similarly 'mixed culture' techniques impose major difficulties in studies on mushroom genetics, physiology and disease. Using the 'Halbschalentest', Eger (1963) showed that the critical change from vegetative (mycelium) to the reproductive fruit-body stage is associated with micro-organisms contaminating the casing soil. Hayes, Rändle, and Last (1969) working with pure cultures of A. bisporus growing in compost and casing soil under aseptic conditions confirmed Eger's findings and isolates of Pseudomonas putida and Pseudomonas Group IV were shown to cause fruit-body formation when introduced to pure cultures of commercial white strains of A. bisporus. The action of Pseudomonas putida was used as a base point for the development of several Petri-plate methods which have been used for investigations on the change which occurs in the growth cycle when the mycelium develops fruitbodies.
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