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Abstract: The introduction of wild species of mushrooms in culture is of great interest both from a practical and theoretical point of view. Some authors have proved that Pleurotus ostreatus Fr. may be successfully cultivated. It was Luthard (1969) who first attempted to cultivate Pleurotus ostreatus in Europe, on stumps and logs of deciduous trees. For inoculation he used a kind of spawn named 'paste' which was made of Pleurotus ostreatus mycelium developed on a medium of myco-sawdust, peptone and malt. Later Toth (1969) cultivated Pleurotus ostreatus on crushed corn cobs, but under sterile conditions. Gyurko (1969) modified that method, growing the same mushroom on corn cobs under non-sterile conditions. For inoculation all authors recommend aerial mycelium as spawn. A technology for cultivation of various species of higher fungi mycelium in liquid nutritive medium (submerged culture) including Pleurotus ostreatus mycelium, has been worked out by Torev (1968). He assumed that the 'liquid spawn' of some mushrooms would be used in mushroom growing. The purpose of the present study was to investigate the conditions and parameters for the cultivation of Pleurotus ostreatus mycelium in liquid nutritive medium and the possibility of its utilization as inoculation material (spawn) for obtaining fruiting-bodies.
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