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Abstract: After a long period of cultivation, the yield of the highly productive strams of cultivated mushroom, Agaricus bisporus, may gradually or suddenly be reduced This can be attributed to either unfavourable environment or genetic variation In our Laboratory, we have studied environmental effects on the agmg of the spawn and also factors affecting the fructification of A bisporus (Huang, 1969, Hou, et al, 1971) under the research projects supported by the Mushroom Reseaich Committee since 1 July 1967 The resultant data indicate the great significance of the study on the genetic make-up of the spawn Therefore our attention has been directed to the genetic deviation of spawn, and it has now become apparent that the control mechanism for so-called degeneration of mushroom largely depends upon the variation in the genetic make-up of the spawn. In the present investigation, we have studied the progenies of (1) basidiospores, (2) secondary spores which were characterized by Kligman (1942), and (3) mycelial fragments obtained from submerged liquid culture which was established by Ivanovich (1965)
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