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Abstract: During the past sixteen years a program of strain selection, development and maintenance has been conducted in the Mushroom Laboratory and Mushroom Research Center of The Pennsylvania State University. Nearly 1,500 monosporous, multisporous, tissue and mycelial transfer cultures have been evaluated. Results of seven strain trials over the past three years are reported. There was no positive correlation between productivity and variety of mushroom (white, cream, or brown), age of cultures, or source of culture (monosporous, multisporous, or tissue). Earlier producing isolates usually yielded the most in an eight-week harvest period. Brown varieties generally produced mushrooms of largest average size with creams and whites following in that order. Average size of mushroom was not correlated with total yield, i.e. both good and poor yielding isolates produced small mushrooms. Spores of A. bisporus collected in 1932 were germinated, providing evidence that these spores can remain viable for at least 36 years. One of the primary purposes for this kind of research is to provide commercial spawn laboratories with cultures recently evaluated and of known high productivity and quality characteristics.
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