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Abstract: A carbon dioxide requirement has been demonstrated for growth of hyphal strands of Agaricus bisporus into sterilised or unstenhsed casing. In sterilised casing the amount of hyphal growth was proportional to carbon dioxide concentration over the range 22 to 370 ppm. At higher levels to 6700 ppm carbon dioxide no further increase in growth rate was observed. Hyphal growth in unstenhsed casing was proportional to carbon dioxide concentration 0-104 ppm. Between 104 and 1000 ppm a retardation of strand growth occurred correlated with production of sporophore initials. Above 1000 ppm carbon dioxide vegetative growth increased and at 6700 ppm was comparable to the maximum rate attained in sterile casing. The carbon dioxide range favouring sporophore initiation was 340 to 1000 ppm. Carbon dioxide was also required for the early stages of sporophore development. No sporophores were produced in sterilised casing. The design of experiments suggests that volatile substances other than carbon dioxide play no part in the process of sporophore initiation in unsterilised casing.
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