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Abstract: Twelve strains of Agaricus bisporus were grown under submerged liquid conditions. Cytological change in mycelium growth in submerged liquid conditions was compared with formation of Kligman's secondary spores in aged agar cultures. Formation and fusion of 'intraphyphal hyphae' were frequently noted Mycelium from submerged liquid conditions was transfered by the syringe method on grain media and planted in mushroom beds Bi-culture technique was developed to determine if hyphae connection in submerged liquid conditions is concerned primarily with food transfer or whether nuclear transfer also takes place.
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