|
Abstract: Among the media commonly used in laboratory cultures of the two-spored mushroom, malt agar and the Treschow medium are most conductive to the germination of Agaricus macrosporus. Temperature requirement for germination is lower than for the two-spored mushroom. More successful germination could be obtained from multispore inocula than from single-spore isolates. The germination power decreased in proportion to the age of the spores. The stimulative effect in the two-spored mushroom, observed by Stanek, was also noticed in the cultures of Agaricus macrosporus. According to the findings of Kehl about the effect of disinfectants and stimulators on the two-spored mushroom, it was ascertained that honey and chloroform treatments could reduce the germination period of A. macrosporus spores with three to eight days. As for the growing conditions, Agaricus macrosporus generally failed to fructify on 16-day horse manure compost without peak-heating. Fructification occurred on synthetic substrates consisting of different kinds of straw, peat and a source of calcium, and opened by sterilization. When, for protein supplementation, by-products of the antibiotic manufacture were added, rapid mycelium growth and rich fructification could be obtained. Soaked substrates of different kinds of straw, peat and/or milling industry refuse gave (after peak-heating) favourable results. According to practice, the 16-day horse manure compost seemed (after proper peak-heating) suitable for cultivation.
Full text download: ISMS subscribers
ISHS members & pay-per-view
Translate:
   |