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Volume 6 Part 1 Article 10
Year 1967
Title: Initiation of Pinheads in Psilocybe panaeoliformis Caused by Certain Bacteria
Author: T. Urayama

Abstract:

Pinhead formation in Psilocybe panaeoliformis is connected with the C/N ratio, via the combined inhibition of an excessive C-source (formation of acids) and an excessive N-source ('inhibitor formation'). Prevention of pinhead formation by the inhibitor is eliminated by the action of Bacillus psilocybe 1. The effect of this bacterium is not due to changes in the pH of the medium to a favourable level, but it seems to be related to a bacterial metabolite acting as an 'anti-inhibitor' or a 'disinhibitor'. This fruit promoting metabolite is dialysable from a watery suspension of the bacterial culture through a cellophane membrane. It is soluble in water, dioxane and alcohol, insoluble in petroleum ether, chloroform, ether and acetone, it is resistant to daylight and to oxidation by air up to 100°C, it is not a reducing sugar, nor a volatile fatty acid, nor a primary or secondary phosphate. It is labile in weak acid or weak alkali solutions. It is probably neither an amino acid, a carbohydrate, an organic acid, adenosine triphosphate sodium salt, gibberellin, IAA, NAA, a vitamin, nor a yeast nucleic acid or a sex hormone.

From 19 g residue of a bacterial culture suspension about 1.7 mg of a crude, active crystalline mass was obtained by separation in cellulose columns.

Pinhead formation was stimulated by the same bacterium in various other species of hymenomycetes.

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