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Abstract: Since the beginning of this century, a number of investigators have reported effects on the growth and development of the commercially cultivated mushroom, Agaricus bisporus (Lange) Sing., caused by volatile substances in the organism's environment. From a review of the literature it is evident that no conclusive investigation had been made to collect or identify the implied metabolic gases of the cultivated mushroom other than cardon-dioxide. Until the present investigation the only gas produced by A. bisporus that had been collected and positively identified was carbon-dioxide. The specific aim of this research was first to determine if the growing mycelium of this fungus produces any volatile substances in addition to carbon-dioxide. In order to establish that any gases collected were produced by mushroom mycelium and were not already present or produced by other organisms, specific controls and pure cultures were essential. The second part of the investigation was concerned with the actual identification of any volatile substances that might be found to be produced by the mycelium. Analytical equipment of recent development was necessary to carry out this second and highly important step.
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