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Abstract: Basic biology and life cycle of morels has established the Sclerotium as the central point in the morel fruiting. Late isolated sclerotia (LIS) have temperature requirements, cold resistance and biochemical properties similar to the sub-terranean mycelial structure connected with the ascocarp in nature. Six culture media viz; Malt Extract Agar (MEA), Potato-dextrose-agar (PDA), Complete Medium+Yeast Extract (CYM), Medium A, Medium B and Czapex's Dox Agar were tested for radial mycelial growth and sclerotia formation with four isolates of M.esculenta. Malt Extract Agar followed by complete Medium+Yeast Extract was found the best culture medium to support the maximum radial growth in all the test isolates. As regards sclerotia formation. Malt Extract Agar medium was the only medium on which all the test isolates of M.esculenta could form sclerotia in plenty. The rest of the culture media though supported radial mycelial growth but failed to initiate sclerotia formation uniformly in all the test isolates of M esculenta. The present study indicates that nutrient poor conditions are not essential for sclerotia production as they could be produced on nutrient rich media also.
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