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Abstract: Heat treatment of Trichoderma species at various stages of their life cycle, confirmed that the chlamydospore structures produced by all the Trichoderma species tested (T. harzianum Thl, Th2, Th3, T; viride, T. longibrachiatum, T. pseudokoningii) had potential to survive temperatures of up to 60°C, for a period of time, in vitro. T. longibrachiatum and T. pseudokoningii isolates proved to be the most enduring, surviving high temperatures as mycelia, conidia and chlamydospores. Unlike some preliminary studies, the chlamydospore stage of T. harzianum Thl did survive in vitro exposure to 60°C, for nine hours. Isolates of Thl, Th3 and T. viride also showed some tolerance to heat exposure of 9 and 24 hours. These results confirm previous findings from our smdies of pasteurized compost, where T. longibrachiatum, T. viride, Thl and Th3 have been isolated more commonly from freshly pasteurized compost than Th2.
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