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Abstract: Latent mushroom (Agaricus bisporus) tyrosinase was activated by a number of methods that result in either conformational changes in the enzyme, or to structural (proteolytic) alterations. Activated enzymes were analyzed with denaturing SDS/PAGE and Western blotting. Kinetic features of both activation process and final active enzymes were characterized. Based on these in vitro observations, a maturation and activation model for mushroom tyrosinase was postulated, that may account for senescence, damage and disease related browning of mushrooms.
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