|
Abstract: The importance of casing bacteria for the pattern of mushroom fruitbody initiation has been smdied. Bacterial populations of different casing materials, such as coir and peats, were found to vary greatly. Of the casing materials tested only activated charcoal and lignite retained an ability to stimulate mushroom primordia formation when sterilized. Isolated casing bacteria (mainly Pseudomonas putida, also an Alcaligenes sp.) were assessed for their effect on mushroom primordia using an agar plate bioassay and microcosms containing cased mushroom spawn. Specific bacterial isolates, identified by ribotyping, were inoculated into microcosms to determine the effect of bacterial dose and timing on primordium formation. The stability and reproducibility of the bacterial effect, after storage by seven methods over 1 year, on initiation is reported. The bacterial effect diminished if isolates were regularly sub-culmred on agar. A molecular marking system (luxAB and tetracycline antibiotic resistance reporter genes) was used to monitor bacteria inoculated into non-sterile casing.
Full text download: ISMS subscribers
ISHS members & pay-per-view
Translate:
   |