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Abstract: Good growth and conidium formation of Humicola insolens Cooney & Emerson on grain was obtained in flasks inoculated with a liquid culture of the thermophilic fungus. Mycelial growth of Agaricus bisporus (Lange) Imbach on grains pre-colonized by H. insolens was better than on sterile grains. Grain pre-colonized by H. insolens and subsequently colonized by A. bisporus (experimental spawn, EGS), provided for better growth of mushroom mycelium on compost than commercial grain spawn (GS). On the 40th day of mushroom growth on compost, the grains of experimental spawn were reduced in width by 25.2% and had lost in weight by 68.2%, whereas these figures for commercial spawn were 4,4% and 41.2%, respectively. A considerable reduction of the nutrients in grains of experimental spawn could prevent the development of weed moulds on grain.
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