|
Abstract: The ability of Pleurotus ostreatus, an edible mushroom of commercial importance, to compete with indigenous straw microorganisms and to colonize non-sterile straw was examined. The presence of the indigenous microorganisms was varied by freating the straw at different temperamres before inoculation with P. ostreatus. Treatment at 22, 60, 90 or 121°C, respectively, inhibited their presence to a different extent and altered the composition of the straw microbial community. The colonization and degradation of the substrate by the white-rot fungus was assessed by measuring the activities of the ligninolytic enzymes manganese peroxidase, manganese-indepedent peroxidase and laccase extracted from the subsfrate. Changes during incubation as indicated by mineralization, amount of water-extractable compounds and pH of the substrate were determined to characterize competition and shifts in dominance of the competitors. The results show that P. ostreatus is able to colonize non-sterile sfraw in smallscale solid subsfrate culmres. Pretreatment of the sfraw at 90° favours colonization by P. ostreatus, whereas treatment at 60°C favours indigenous organisms with particular resistance to P. ostreatus. From the three investigated enzymes the course of MnP activity is possible best suited to show a delay in the fiingal development due to competetion with indigenous microorganisms.
Full text download: ISMS subscribers
ISHS members & pay-per-view
Translate:
   |