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Abstract: More than 20 species of thermophilic fungi were found at the end of the composting process in the substrate. Growing of A. bisporus under confrol conditions in a growing room changed the quality and quantity of microfungi in the compost. In a smdy aimed to investigate those changes, compost samples were taken during the growing cycle of A. bisporus in a commercial mushroom farm. After 7 days incubation of the samples on sterile, wet filter paper and malt agar, isolation and identification of fungi was canied out. The following species were identified: Acremonium sp., Chaetomium olivaceum, Trichoderma harzianum, Mucor mucedo, Rhizopus stolonifer, Epicoccum purpurascens, Trichotecium roseum, Chromelosporium stage of Peziza ostracoderma, Coprinus sp. and Doratomyces stemonitis. All of the species identified, except for Chromelosporium sp., are very common and their isolation from soil, air, water, live and dead plant material etc., can be done routinely. However, Chromelosporium sp., according to the literature data, is specific for A. bisporus growing rooms.
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