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Abstract: One of the major problems in the cultivation of oyster mushrooms (Pleurotus ostreatus) is the enormous production of spores by the fruitbodies, amounting up to several hundreds of millions of spores per gram of fruitbody tissue per day Many workers in the production of these mushrooms develop an allergy with symptoms similar to an "extnnsic allergic alveolitis (EEA)" For the development of a sporeless sfrain of P ostreatus we used sfrain ATCC 58937 This non-commercial sfrain is completely sporeless We have recovered both nuclear types of stram ATCC 58937 as monokaryons (hereafter refened to as nhl 1 and nh42) by protoplasting the mycelium. Our smdies indicate that sporelessness in sfrain ATCC 58937 is linked to a limited number of loci, present in both nuclear types The exact number of loci remains to be determined This is in contrast with previous reports which state that sporelessness is a dominant trait present only in nuclear type nh42 Our smdies also show that sporelessness is not linked to the unfavorable appearance of the fruitbodies This information provides a good basis for the development of a sporeless strain of P ostreatus To accelerate our efforts in developing this strain we are cunently working on the development of molecular markers linked to sporeless trait As shown m our preliminary map one of these markers is linked to one of the loci involved in sporelessness
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