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Abstract: Volvariella volvacea, commonly known as the Chinese straw mushroom, is an important edible fungus of the tropics and subtropics. It grows preferentially at high temperature and very high relative humidity. Contrary to other edible fiingi, this mushroom will lyse and die when the ambient temperature decreases to 4°C for a short period of time. Because V. volvacea has a short shelf-life and cannot be stored at low temperature, commercial distribution has been limited. To smdy the straw mushroom's sensitivity to low temperature we used RNA differential display to isolate several cold-shock genes induced by a temperature drop to 4°C. Amplified coldshock related cDNA fragments were cloned and sequenced. A genomic DNA library was constructed with a Lambda GEM-11 genomic cloning vector. Cold-shock related clones were isolated from the library and further characterized.
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