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Abstract: A wild population of Agaricus bisporus has been discovered in Greece Collecting among sheep manure or under Cupressus sempervirens allowed us to isolate more than 100 strains either by tissue culture of sporocarps, or by spore germination, or from Cupressus litter Morphological, biological, and genetic analyses revealed high vanabihty The cap colour vaned from brown to white. About 10 % of the strams produced white sporocarps with L values ranging between 89 and 92 The average spore number per basidium was generally near two, indicating a preponderantly pseudohomothalhc hfe cycle An exception was the strain Bs 514, producmg tetrasponc sporocarps. Genotypic analysis at three alloenzymatic markers revealed 37 different genotypes, mcludmg new alleles Allelic frequencies differed from those observed in population samples from California, France, or Russia Because inhentance of the smdied traits has previously been elucidated, we believe that this highly diverse population will be useful for further breedmg work
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