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Abstract: We have developed a stable transformation system in Pleurotus ostreatus using a homologous drug resistant marker gene, CbxR. Here we present coexistence of a single strand carrier DNA enhanced the efficiency of transformation by up to 50-fold. About 200 transformants per pg of vector plasmid were obtained with the modified protocol, which is the highest transformation efficiency for edible mushrooms reported to date. Using the transformation system, we also carried out overexpression of recombinant manganese peroxidase (mnp) genes in P. ostreatus. Enhanced enzyme activity was observed in the early stage of liquid culture of the transformants, indicating that the transformation system works as an efficient tool for the molecular breeding of the mushroom.
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