An RT-PCR test for the presence of one of the La France disease-specific dsRNAs in pure cultures of Agaricus bisporus, fruit bodies, spawn and spawnrun compost was optimized. A sensitive and reliable test is now available for the detection of viral dsRNA at any stage of the cultivation of mushrooms. A collection of wild isolates of A. bisporus was tested for the presence of disease-specific viral dsRNAs. No dsRNAs were found. DsRNAs could be transferred repeatedly from one commercial line to another by hyphal anastomosis. Attempts to transfer dsRNAs from an infected commercial line to six wild isolates, however, were not successful.
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