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Abstract: Pharmacological effects of the mycelial extract of cultured Cordyceps sinensis (Cs) on rat organs were examined, and compared with those of the Chinese medicine of Cs. The Chinese medicine of C. sinensis is referred to as Cm. Both kinds of extracts inhibited twitch contractions of the trachea in a dose dependent manner. The IC50 value for Cs was about 10 pg/ml, but Cm was 6-8 times weaker than Cs. This inhibition of twitch contractBwas not affected by 8-phenyltheophylline, ATP, phentolamine and DL-propranolol. The both extracts of Cs and Cm at 20 pg/ml, and theophylline (10 µM) relaxed the trachea which was contracted by 50 mM K+. Cs was most potent in relaxation . The extract of Cs also relaxed the K+ -evoked contraction of aortae. The active fraction of the Cs extract was eluted below the molecular weight of lOOODa by Superdex 30 PG (Pharmacia) gel filtration. This fraction caused 8-phenyltheophylline-resistant inhibition of twitches of the trachea. These results indicate that the mycelial extract of cultured Cs produces non-purinergic and non-adrenergic inhibition of tracheal twitch contractions and that the extract relaxes the persistent contraction of the trachea and the aorta. The present study demonstrates that the extract of cultured Cs is more effective in reducing tracheal activities than that of Cm.
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