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Abstract: Because of environmental legislation in the Netherlands, phase I of composting will be performed indoor in tunnels starting in 1995. High temperatures are easily created and maintained in the whole compost bulk. This puts survival of Scytalidium thermophilum at risk. The presence of this thermophilic fungus is required for compost selectivity, in particular for successful growth of Agancus bisporus mycelium. It occurs naturally in compost ingredients and survives traditional phase I in windrows. Inoculation in between phases I and II has to be considered. Calculated estimates and empirical data are presented on the required amounts of inoculum of either phase II compost, full of S. thermophilum, or of a selected S. thermophilum isolate.
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