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Abstract: To determine phylogenetic relationship of strains of Pleurotus and allied genera, we amplified partial 18S ribosomal RNA gene (1 SSrDNA) and internal transcribed spacer region (ITS) by polymerase chain reaction and then sequenced. We compared 383 (384 in some species) bases of 1 SSrDNA (1341 to 1722 in Saccharomyces cerevisiae 1 SSrDNA) and 179 bases of ITS-1 (between ISSrRNA gene and 5.8SrRNA gene), and drew phylogenetic trees of Pleurotus. The 1 SSrDNA was very conservative, and similarities of 1 SSrDNA among all species we examined were more than 90 %. The phylogenetic tree showed Pleurotus was separated from other genera we examined. In addition it was shown that Pleurotus tuberregium (Fr.) Singer (P. tuberregium sometimes has been placed on Lentinus or Panus) was distantly related to Lentinus and Panus. In the ITS-1 there were many insertions and deletions. The ITS-1 of P. pulmonarius was very close to that of P. ostreatus.
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