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Abstract: In order to define targets for strain improvement of Agaricus bisporus it is essential to collect knowledge on the physiology and biochemistry of the various stages of the life cycle. However, classical genetics of this organism has not been extensively studied whereas genetic manipulation is still at its infancy. Molecular characterisation of highly expressed genes involved in basic metabolism therefore provides important information on the gene structure of A. bisporus and basidiomycetes in general and allows us to study strong promoters that can be used for studies on transformation and expression of genes of interest in this fungus. Cloned genes can also be used as genetic markers for the constmction of a genetic linkage map and for comparison of the commercial isolates with wild-collected isolates using restriction fragment length polymorphism. We present data here on the gene-stmctures of several (key) genes involved in ammonia assimilation, glycolysis and the pentose phosphate pathway.
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