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Abstract: In order to expand our knowledge of the process of fruit body development of Agaricus bisporus, we are studying genes which show no or low expression in the vegetative growth phase but are highly expressed during fruit body formation. For this purpose we have constructed a cDNA expression library of pinheads of A. bisporus strain Horst®Ul and we have screened this library using Northern hybridization techniques for genes which show elevated steady state mRNA levels in pin-heads and/or mature fruit bodies. In this way we have isolated several clones which show at least a 100-fold increase of steady state mRNA levels in fruit bodies compared to mycelium. By sequence analysis of these fruit body induced genes we have identified a gene induced during fruit body formation encoding a 5- subunit of the mitochondrial ATP-synthase complex. The genomic structure of the genes and their flanking regions will be further characterized and also promoter studies of these highly inducible genes will be performed. Another application of the cloned genes is their use as polymorphic markers for the development of a genetic linkage map.
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