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Abstract: The best linear growth of five strains of Agaricus brunnescens Peck was obtained on malt extract agar at 25°C. Starch and peptone proved best carbon and nitrogen sources. Different concentrations of lime and gypsum alone and in combination with wheat grain showed that 6% gypsum and 8% lime alone and 2% gypsum and 6% lime in combination proved suitable for spawn production. Sterilization gave better results among different methods tried. Among the three recipes of synthetic composts tried, A3 showed maximum spawn running.
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