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Abstract: The cultivated mushroom, Agaricus bisporus, is particularly recalcitrant to genetic manipulation and other laboratoiy investigations. Studies of enzyme mutants to understand the role of altered production of extracellular enzymes in substrate utilisation and fruiting of A. bisporus is of considerable significance but mutants are not readily generated in this species. We have chosen another secondarily homothallic basidiomycete, Coprinus bilanatus, in which to produce enzyme mutants. This fungus is genetically similar to A. bisporus and has typical basidiomycete features such as, a regularly clamped binucleate dikaryon and a uninucleate monokaryon. Both mycelial types produce asexual spores (oidia). Moreover, this fungus fruits readily in vivo and its basidiospores germinate rapidly. The production, characterisation and properties of some mutants responsible for cellulase, xylanase and amylase production will be reported.
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