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Abstract: Growing of Pleurotus spp. as mushrooms JS becoming important throughout the world because of their capabilities to grow at wide range of temperatures (20- 35°C) and on vanous lignocelLuloses. The commercialLy cultivated species of Pleurotus are the oyster mushroom P. ostreatus, the phoenix mushroom P. sajorcaju, and others like P. cornucopiae, P. sapidus, P. sp. cfr. Florida and P. eryngn. Zadrazil and Grabbe (1983) reported a world production of 40,000 tons annually of Pleurotus spp. Recently it gained popularity in India (Khanna and Garcha 1984) and Canada (Pidgeon and Anderson 1981; Mueller and Gawley 1983). Production of mycelium of mushroom, Agaricus spp. in submerged culture was started as early as 1949 (Humfeld and Sugnhara 1949). Later on, mycehum of morel and other mushrooms was produced by other researchers (Chahal 1984; Litchfield 1967a, MarUn 1982; Zadrazil 1978). The mycelium of Pleurotus spp. has been produced in submerged cultures by vanous researchers (Buckhalo and Solomko, 1978; Chahal, 1984; Fnednch et al., 1986; Hadar and Cohen-Arazi, 1986; Zadrazil, 1978) on vanous substrates (beer wort, sulfite liquor from wastes of paper manufactunng, hemicellulose fraction, glucose, sugar beet molasses and apple dnstillery waste). Even fruiting bodies of P. sapidus have been produced on alfalfa-brown-jmce by Kurtzman (1978). But there as no other report of mycelium production of P. sajor-caju on lignocelLuloses except that by Chahal (paper communicated). Pleurotus sajor-caju is one of the fungi used in our process, "An Integrated Process for Production of Food, Feed and Fuel (Ethanoi) from Biomass" (Chahal 1984) to produce fungal biomass in submerged culture on lignocellulosac material, com stover, to be used as a protein supplement in animal feed. P. sajor-caju was selected because i t has already been accepted as human food in the form of mushrooms and no toxic materials are known to be produced by it.
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