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Abstract: During the substrate preparation for the growth of Agaricus bisporus, polysaccharides of plant and microbial origin, and nitrogen compounds allow the biological activity of microorganisms. Simultaneously, the deposition of dark,humus-like material occurs in which polysaccharides, lignin, nitrogen compounds and polyphenols condense. The rapid disappearance of this material after spawning suggested that it could be an important nutrient source during the period of active growth of the mycelium(Sinden and Hauser, 1953), even though its chemical nature and evolution are only partly understood. This work reports a study of the characteristics of this humus-like material and its changes during Phase I and Phase 2 of composting. Changes of extractable quantities and molecular sizes and the characteristics of the absorption spectra in parallel to the evolution of the main'classes of compounds were recorded.
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