Development of in Vitro Root System from Microcuttings of Fruit Trees
Takuya Tetsumura, Kazumi Irishima and Chitose Honsho
Success of micropropagation (in vitro propagation) depends on the establishment of four stages: establishment of explants, multiplication of shoots, rooting of microcuttings, and acclimatization of micropropagules to ex vitro environment (Murayama et al., 1989). During the acclimatization, loss of micropropagules directly affects multiplication rate. Hence, there are many reports aiming to prevent them from wilting by controlling the humidity, light, carbon dioxide, and so on. However, most microcuttings of fruit trees, that is, woody plants, are difficult-to-root and need treatment with auxins in order to root. A treatment with a high concentration of auxin often results in the weakening of microcuttings, and many of them do not survive during acclimatization even if they have roots and grow in a suitable environment.
Japanese persimmon (Diospyros kaki Thunb.) is one of the fruit trees whose micropropagules are difficult to acclimatize (Tao and Sugiura, 1992).
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