Vol. 56

Title:
Hygiene Problems in Plant Tissue Culture Propagation

Author:
Sridevy Sriskandarajah

pp: 238-241

Abstract:
INTRODUCTION

Plant tissue cultures have been used for various purposes such as mass propagation, production of disease-indexed plants, and genetic engineering. Plant tissue culture is defined as culture of plant cell, tissue, or an organ under sterile conditions in an artificial medium under aseptic conditions. This statement clearly indicates that for a successful tissue culture operation there should not be any contamination by microorganisms during the entire culture period. Contamination by microorganisms will not allow the plant cultures to grow, and the contaminants will eventually destroy plant cultures. Therefore, it is important to be aware of the major contaminants, the sources of such contaminants, and the methods available for sterilizing plant material before the introduction into tissue culture medium and to know the methods for an overall hygiene in a tissue culture operation.

Contamination caused by some common microorganisms can result in large losses during

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