Vol. 55

Title:
Production of Disease-Resistant Tissue Culture Seedlings Using Endophytic Actinomycetes

Authors:
Hitoshi Kunoh, Tomio Nishimura, Sachiko Hasegawa, Akane Meguro and Tadao Fujimori

pp: 212

Abstract:
In the mid 1990s several researchers found that a number of actinomycetes inhabit a wide range of plants as either symbionts or parasites. Since then, endophytic actinomycetes have been attractive sources of novel antibiotics and growth regulators of other organisms. Actinomycetes, especially Streptomyces species, isolated from the rhizosphere have proven to be excellent biocontrol agents of soilborne plant pathogens. Such an effective activity is largely dependent on secondary metabolites produced by these organisms.

These earlier reports led us to assume that if a useful endophytic actinomycete isolated from a field-grown plant can successfully colonize tissue-cultured seedlings of the plant, the seedlings could become resistant to various plant pathogens. Because tissue culture flasks are usually axenic, such a novel technique should allow this actinomycete to colonize easily its host plant without competition and/or antagonism by any other microbes.

Twenty-five strains of endophyt

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