In Vitro Propagation of Cryptocoryne Species
Tomoya Araki and Hisashi Harada
In vitro propagation protocol for Cryptocoryne species is reported. Shoot proliferation of C. wendtii and C. pontederiifolia were promoted on Murashige and Skoog (MS) agar solidified medium supplemented with BA at higher concentrations (1 to 20 µM). Compared to solid medium, shoot proliferation was improved in liquid-shake culture. However, abnormal shoot growth was observed in both species. In C. pontederiifolia, shoot grown in liquid-shake culture showed chlorotic leaf growth. In C.wendtii, leaves were needle-like in appearance. A double-layer culture method gave high yield of normal and healthy shoots. The volume of the additional liquid medium in double-layer culture affected shoot proliferation.
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