Micropropagation and Growth Regulation of Salvia greggii Variegated
Sinead Phelan and Alan Hunter Gerry Douglas
Micropropagation and growth regulator treatments have the potential to produce dense, compact Salvia greggii variegated commercially. We identified suitable media for shoot proliferation and rooting and studied the effects of paclobutrazol and gibberellic acid on shoot and internode length.
We achieved 100% rooting with explants cultured on MS medium supplemented with 1.0 mg·L-1 IBA. Survival of explants on weaning was 93% to 100%. High concentration of paclobutrazol in vitro reduced both the internode length and number of nodes produced per shoot. After weaning, treated shoots were not significantly shorter than those untreated.
For glasshouse-grown plants, paclobutrazol drenches significantly reduced both plant height and internode length and increased bud break but did not affect the number of nodes produced. The maximum loss of variegation was 19% in all treated plants.
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