Propagation and Acclimatization of ‘Norton’ Grapevine
Paul E. Read and Brant B. Bigger
Difficulty is often experienced by commercial propagators in propagating the ‘Norton’ grapevine (Vitis aestivalis, also known as ‘Cynthiana’) by cuttings, the normally employed method of grapevine multiplication. Therefore, we endeavored to propagate ‘Norton’ by micropropagation. Cultures were readily established in vitro by placing axillary buds taken from 3-year-old greenhousegrown potted vines on Murashige and Skoog medium supplemented with 4 µM benzyladenine (BA) and 0.5 mg thiamine per liter. When in vitro-derived axillar-derived axillary buds were cultured on various levels of BA and naphthaleneacetic acid (NAA), excellent multiplication was obtained when 4 to 8 µM BA was included in the medium. Naphthaleneacetic acid had little effect on shoot number or number of nodes per shoot. Plantlets were simultaneously acclimated and rooted ex vitro in rehydrated compressed peat pellets (Jiffy-9). Rooting was nearly 100% and over 90% of rooted microcuttings were successfully established in the greenhouse and later transferred to the field.
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