Acclimatizing Tissue Culture Plants: Reducing the Shock
John F. Seelye, Garry K. Burge and Ed R. Morgan
Many tissue culture milestones have been achieved over the past 50 years (Gamborg, 2002), with micropropagation established as a commercially viable form of vegetative propagation since the 1970s. Although many species are propagated using this technology there are still many more species that are either recalcitrant or cannot be cost-effectively propagated by tissue culture. Consequently, the commercial application of tissue culture propagation is restricted to mass propagating, high value, superior genotypes, and/or high health lines.
Plant tissue culture relies on growing microbe-free plant material in a sterile environment, in conjunction with defined media containing nutrients, growth promoters, and a carbohydrate source. Typically plant tissue culture is carried out on
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