Somatic Embryos Produced From Aseptic Seedlings of Wild Cyclamen Species
Kazumi Furukawa, Fumika Kakihara and Masahiro Kato
Somatic embryos were produced using aseptic seedlings of wild Cyclamen species.
The seeds of 18 wild Cyclamen species were cultured in the dark at 15 to 20°C until
germination, and then maintained under a 16/8-light/dark photoperiod at 24°C.
Modified Murashige and Skoog medium supplemented with 30 g · liter-1 sucrose
and 3.0 g · liter-1 Gellan gum was used for culture of aseptic seedlings. The seeds of C. balearicum, C. creticum, C. intaminatum, and C. repandum did not germinate.
Aseptic seedlings were obtained from 14 Cyclamen species: C. africanum, C. cilicium,
C. coum, C. cyprium, C. graecum, C. hederifolium f. album, C. libanoticum, C.
mirabile, C. parviflorum, C. persicum, C. pseudibericum, C. purpurascens, C. rohlfsianum,
and C. trochopteranthum. Sectioned petioles and leaves from the seedlings
were inoculated on two types of induction media. The induction was carried out in
the dark at 24°C. Among the 14 species, only C. creticum, C. libanoticum, and C.
parviflorum did not produce
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