Effect of Complex Supplements on Growth of Ophrys Seedlings in Vitro
Mature seeds of Ophrys apifera, O. fusca, O. lutea, and O. sphegodes were surface-sterilized and immediately sown in vitro. Germination occurred on all tested media. Among eight tested media, Norstog medium gave the highest germination percentage for these four Ophrys species. The effect of complex supplements (organic nitrogen sources) on germination of O. apifera was tested. Among the four complex supplements tested, organic supplements of Norstog gave the highest germination percentage.
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