In vitro Inoculation Test for Resistance to Crown Gall Disease on Roses
L. Zhou, H. Fukui and S. Matsumoto
Rosa ‘Fashion Parade’, R. canina, R. canina ‘Superbe’, R. ‘Pekcougel’, Anna® hybrid tea rose, and R. ‘Meihartfo’, Kalinca® floribunda rose (syn. R. ‘Pink Wonder’) were cultured by shoot tip culture and were micropropagated every 6 weeks in vitro. Agrobacterium tumefaciens was isolated from crown gall collected from a rose plant. The shoots inoculated with A. tumefaciens formed white or white-green crown galls. Four methods were used for inoculation: (1) needle prick inoculation (needle), (2) spread inoculation at upper end of shoot (upper), (3) spread inoculation at lower end of shoot (lower), (4) slice off bark and inoculate (slice). Shoot growth was not affected by inoculation of A. tumefaciens except for slice. Four weeks after needle inoculation, the rate of shoots which formed crown gall rose to 70% and remained stable. Therefore, the needle prick inoculation was the most successful and easiest to administer.
Five roses: R. ‘Fashion Parade’, R. canina, R. canina ‘Superbe’, R. ‘Pekcougel’, Anna® hybrid tea rose, and R. ‘Meihartfo’, Kalinca® floribunda rose were inoculated by the needle method. Rosa canina and ‘Fashion Parade’ had no resistance to infection and disease. Rosa canina ‘Superbe’ was resistant to infection but lacked resistance to disease. Rosa ‘Pekcougel’, Anna® hybrid tea rose, and R. ‘Meihartfo’, Kalinca® floribunda rose (syn. R. ‘Pink Wonder’) were not infected and resisted disease infection.
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